fluorescein fitc conjugated avidin  (Vector Laboratories)

Journal: PLoS Pathogens

Article Title: The Gag Cleavage Product, p12, is a Functional Constituent of the Murine Leukemia Virus Pre-Integration Complex

doi: 10.1371/journal.ppat.1001183

Figure Lengend Snippet: U/R cells were infected with the 1xMycR virus and analyzed by immunofluorescence, as described in . p12 (A–C), MA(A, B) and CA (C) were detected with the anti-Myc monoclonal antibody, goat anti-MA and goat anti-CA polyclonal antibodies, respectively, and the matched secondary FITC-conjugated donkey anti-mouse IgG and Red-X- conjugated donkey anti-goat IgG. For consistency, the images were pseudocolored to present p12 in red and MA or CA in green. Dashed lines mark the boundaries of cell-free regions where virions were detected attached to the cover glass. Bars represent 10 µm.

Article Snippet: The hybridized biotin-labeled probe was detected with FITC-conjugated avidin (A-2011, Vector Laboratories,1∶400 dilution), which was incubated with the slides for 30 min at 37°C in 1% BSA/4xSSC and 0.1% Tween 20.

Techniques: Infection, Immunofluorescence

Journal: PLoS Pathogens

Article Title: The Gag Cleavage Product, p12, is a Functional Constituent of the Murine Leukemia Virus Pre-Integration Complex

doi: 10.1371/journal.ppat.1001183

Figure Lengend Snippet: U/R cells (A–H) or U20S (I) were infected with 1xMycR (A–G, I) or wt (H) viruses, and 12 h (A–E, G–I) or 2 h (F) postinfection the cells were processed for immunofluorescence and FISH analysis. Myc-labeled p12 proteins were detected with a primary anti-Myc monoclonal antibody (A–F, H–I) and a secondary Cy-3-conjugated anti-mouse antibody (A–I). The immunodetection in G was performed with only the secondary, but not the primary, antibodies. The genomic DNA was detected with MLV-derived biotin-labeled probes and a FITC-conjugated avidin (A–I). D and E are magnifications of the insets in A and B, respectively. Arrows indicate overlaps between the viral p12 proteins and the genomic DNA signals. Ellipses and triangles show non-overlapping signals of the p12 and the genomic DNA, respectively. Images A, B, D, E and G–I were taken with a BX50 microscope (Olympus) or with a LSM 510 META confocal microscope (Zeiss) (C and F). Bars represent 10 µm.

Article Snippet: The hybridized biotin-labeled probe was detected with FITC-conjugated avidin (A-2011, Vector Laboratories,1∶400 dilution), which was incubated with the slides for 30 min at 37°C in 1% BSA/4xSSC and 0.1% Tween 20.

Techniques: Infection, Immunofluorescence, Labeling, Immunodetection, Derivative Assay, Avidin-Biotin Assay, Microscopy